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KMID : 0665220180310040457
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Korean Journal of Food and Nutrition
2018 Volume.31 No. 4 p.457 ~ p.463
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Study on the Protective Mechanism of Vitamin C in the SH-SY5Y Cell Death Induced by the Streptozotocin
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Lee Seung-Hee
Han Kyung-Hoon
Kim Hyun-Jun
Park Kwang-Sung
Han Sung-Hee
Kim Jung-Hee
Heo Jae-Hyeok
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Abstract
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In this study, we analyzed the protective effects of the vitamin C in the streptozotocin (STZ)-induced apoptosis using the SH-SY5Y, a neuroblastoma cell line. The cells were pretreated with the vitamin C (100 μg) for 30 min, followed by the 24-hr treatment with the 2.5-mM STZ. The cell-viability assay using the Cell Counting Kit (CCK)-8 revealed the cell-survival rate increased by 15% following the vitamin-C pretreatment compared to the STZ-only treatment. Moreover, we conducted the western-blot analysis to determine the protective effect of the vitamin C regarding the apoptosis. Compared to those in the STZ-only-treatment group, the p-ERK and Bcl-2 expressions increased in the vitamin-C-pretreatment group, whereas the p-JNK and Bax expressions decreased. The vitamin-C pretreatment increased the expression of the SOD-1, an antioxidant enzyme, by more than 30%, indicating its protective role in the STZ-induced oxidative stress. Also, we found both the intrinsic- and extrinsic-pathway mechanisms of the STZ-induced apoptosis. The results of this study sμggest vitamin C may help prevent the neurodegenerative diseases.
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KEYWORD
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Alzheimer’s disease, streptozotocin, reactiveoxygen species, vitamin C
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